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Home » Seminar » Seminar Topics

Expression and Regulation of Protein Complexes in Yeast.

Expert Suggestions on Preparation for Computer Based CAT-09

Seminar

1 Structural Biology and Centre for Computational Biology, Hospital for Sick Children, 555 University Avenue, Toronto, M5G 1X8, Canada 2 Service de Conformation de Macromolecules Biologiques, Centre de Biologie Structurale et Bioinformatique, Université Libre de Bruxelles, CP263, Blvd. du Triomphe, 1050, Bruxelles, Belgium Multiprotein complexes play an essential role in many cellular processes. But our knowledge of the mechanism of their formation, regulation and lifetimes, is very limited. Timely transcription of the corresponding genes is one mechanism by which protein complexes are regulated. We investigate this mechanism in Multi-protein complexes in yeast by mapping gene expression data onto the manually curated set of protein complexes deposited in the MIPS database. Rather than combining all the available gene expression data, which includes data measured under more than 500 different conditions we examine the expression profiles of genes corresponding to components of individual complex under different sub-groups of conditions independently. In a previous study we identify putative regulatory sequence motifs in the upstream regions of the genes involved in individual complexes, and predicted groups of co-regulated genes in each complex on the basis of these motifs. Combining the condition dependent gene expression analysis with the identified putative regulons, allows us to obtain a dynamic picture of the conditions dependent transcriptional regulation program of protein complexes in yeast.

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